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AdipoGen Life Sciences
anti-Notch1 (mouse), mAb (22E5)
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Product Details | |
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Synonyms | Neurogenic Locus Notch Homolog Protein 1; Motch A; mT14; p300 |
Product Type | Monoclonal Antibody |
Properties | |
Clone | 22E5 |
Isotype | Rat IgG2aκ |
Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
Immunogen/Antigen | Recombinant mouse Notch1:Fc (AG-40B-0109). |
Application |
Flow Cytometry: (1:500) Immunofluorescence: (1:1000) (see Reference 1) |
Crossreactivity | Mouse |
Specificity |
Recognizes endogenous mouse Notch-1 receptor. |
Purity | ≥95% (SDS-PAGE) |
Purity Detail | Protein G-affinity purified. |
Concentration | 1mg/ml |
Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
Isotype Negative Control | |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS |
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Product Specification Sheet | |
Datasheet |
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Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.
- Dynamic Regulation of Notch 1 and Notch 2 Surface Expression during T Cell Development and Activation Revealed by Novel Monoclonal Antibodies: E. Fiorini, et al.; J. Immunol.183, 7212 (2009)
- Cutting edge: Thymic NK cells develop independently from T cell precursors: V.S. Ribeiro, et al.; J. Immunol. 185, 4993 (2010)